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Dear Scientists, LAMP, short for loop-mediated isothermal amplification, is an alternative technique/method for DNA amplification and has some advantages over PCR. The principle of the LAMP assay is based on a selection of 4 to 6 primers as shown as in this animated video*. Best Henrik *Animation was done with Microsoft Office Powerpoint. References: 🤍 Time Codes: 0:00 Introduction 0:37 LAMP vs PCR 1:19 LAMP principle 3:47 Outro
Did you know that this isothermal amplification method can be performed in as little as 5-10 minutes with limited resources? Learn more at 🤍 0:00 - Designing a LAMP Experiment 0:12 - Strand Displacement and Initiation of Amplification 0:24 - Synthesis: Loop Formation 0:42 - LAMP Dumbell Structure Formation 0:54 - LAMP Amplification
This video lecture explains about Loop Mediated Isothermal Amplification. Related Videos: - Plotting Bacterial Growth Curve: 🤍 Colony Forming Units: 🤍 Serial Dilution Methods: 🤍 Copy Number Calculation for qPCR: 🤍 How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: 🤍 How to generate qPCR standard curve in excel and calculate PCR efficiency: 🤍 Real Time qPCR optimization, Calculating PCR Efficiency: 🤍 Taqman Assay Vs SYBR Green Assay: 🤍 Resolving poor PCR efficiency: 🤍 Calculating molecular weight of Unknown protein 🤍 Designing qPCR Primers 🤍 Oligonucleotide Preparation & Purification 🤍 How to check Oligo Concentration 🤍
Loop Mediated Isothermal Amplification-LAMP is a updated version of PCR technique. In this video we tried to describe its mechanism, components easiest way to understand.
See our LAMP solutions: 🤍 Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that is rapid, sensitive, accurate, and cost-effective. Due to the isothermal amplification characteristics, LAMP is amenable to assaying diagnostic samples at the point-of-care or environmental samples at the point-of-collection. Over the years scientists have used LAMP technology to develop assays for a variety of infectious agents including bacteria, viruses, fungi and parasitic organisms. They also developed assays for the diagnosis of cancer, identification of invasive species, detection of food adulteration and identification of drug resistance, among others. However, assay development for new targets remains challenging in part due to the complex design requirement of six LAMP primers and the need to optimize buffer conditions. Suboptimal assay design results in slow amplification kinetics, low sensitivity, and poor specificity. This webinar will outline the basic design of LAMP assays and how it is different from PCR. We will discuss key parameters that affect assay performance and approaches to assay optimization. We will also detail common methods to monitor amplification and how to troubleshoot assay failures. Lastly, we will introduce a novel thermostable enzyme suitable for robust and sensitive DNA as well as RNA LAMP. Key Learning Objectives Learn basic design of LAMP assays and the key differences from PCR Learn key parameters that affect assay performance and approaches to assay optimization Explore the common causes of assay failure and how to troubleshoot Discover ways to apply LAMP in point of care/point of collection using a novel thermo-tolerant LAMP master mix that is room temperature stable when freeze dried
HiberGene's Molecular Assays employ the novel LAMP technology.
With the 3M™ Molecular Detection System you can be confident that your pathogen testing is accurate and reliable. Save time and labour costs with ready-to-use reagents, a single protocol for all pathogens and results in minutes. Plus the ability to test for multiple types of pathogens simultaneously. Learn more: 🤍
Watch the Full Video at 🤍 A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci - a 2 minute Preview of the Experimental Protocol Simon Blaser, Hanspeter Diem, Andreas von Felten, Morgan Gueuning, Michael Andreou, Neil Boonham, Jennifer Tomlinson, Pie Müller, Jürg Utzinger, Beatrice Frey, Jürg E. Frey, Andreas Bühlmann Agroscope, Department of Method Development and Analytics; Swiss Tropical and Public Health Institute,; University of Basel,; Federal Office for Agriculture, Swiss Federal Plant Protection Service; OptiGene Limited,; Fera Science Limited,; Newcastle University, School of Natural and Environmental Sciences; Agroscope, Department of Plants and Plant Products; This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports. Visit 🤍?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: 🤍
The International Potato Center has validated a field-deployable Loop-mediated Isothermal Amplification (LAMP) assay for detection of Ralstonia solanacearum spp., from stem, tuber, and soil samples without extensive resources. In this video, we take you through the steps on how to use this novel method for Ralstonia solanacearum diagnostics directly in farmers' fields.
A simple animation demonstrating LAMP, let me know if you'd like some commentary as I could make a second version. Credit to Shaïma Azouzi for the animation and graphics. LAMP is a kind of polymerase chain reaction (PCR) like amplification protocol. For the detection of specific DNA sequences in a sample. It produces binary results, present or not present. It uses 4 primers which recognise 6 regions, thus its amplification is much more specific and more sensitive than classic PCR. It uses BST polymerase for this which presents a strand displacement property allowing it to amplify at a stable temperature, and without need for hybridisation/dehybridisation processes.
تم نشره 8\4\2018 Loop-mediated isothermal amplification (LAMP) is a simple method that can amplify DNA with high specificity, sensitivity, and rapidity LAMP method is faster. It is basically an isothermal amplification process unlike PCR but makes use of 4-6 primers which consist of outer primers and inner primers with or without loop primers. It utilizes the strand displacement ability of Bst polymerase and hence gives rise to a loop product which gets amplified to give various sized final products
Biotek-M™ Dengue Aqua Kit is a diagnostic kit to rapidly detect dengue infections in clinical samples. This employs the isothermal platform Loop-mediated Isothermal amplification (LAMP) and amplifies Dengue RNA with high specificity and efficiency.
Air quality monitoring using Loop-Mediated Isothermal Amplification (LAMP) PCR is an emerging and promising approach to assess airborne microorganisms and pollutants, providing valuable insights into environmental health and public well-being. LAMP PCR offers several advantages for air quality monitoring, including its rapidity, sensitivity, and ability to be deployed in various settings. One example of LAMP PCR in air quality monitoring is the detection of pathogenic bacteria in indoor environments. Indoor air quality can significantly impact human health, especially in places like hospitals, offices, and schools. LAMP PCR allows for the quick detection of harmful bacteria, such as Legionella, which can cause severe respiratory infections. By identifying the presence of such bacteria, mitigation strategies can be promptly implemented to improve indoor air quality and reduce the risk of infections. Another application of LAMP PCR in air quality monitoring is the assessment of allergenic fungal spores. Fungi like Aspergillus and Penicillium release spores into the air, leading to respiratory allergies and asthma in susceptible individuals. LAMP PCR can rapidly detect the presence of these specific fungal species, aiding in the early warning and management of indoor and outdoor environments where spore concentrations are high. LAMP PCR is also useful for monitoring airborne viruses. In the context of the COVID-19 pandemic, LAMP PCR has been explored as a potential tool for rapid and on-site detection of SARS-CoV-2 in indoor and outdoor air. This application can assist in assessing viral transmission risks in public spaces and healthcare facilities. Furthermore, LAMP PCR can be applied to monitor changes in microbial communities in response to air pollution or environmental disturbances. By targeting specific genetic markers of key microbial species, researchers can gain insights into ecosystem health and dynamics. Overall, air quality monitoring using LAMP PCR has the potential to revolutionize our understanding of the microbial composition of the air and its impact on human health and the environment. By complementing traditional air quality monitoring methods, LAMP PCR contributes to comprehensive and data-driven strategies for air pollution management and public health protection. #AirQualityMonitoring #LAMPPCR #EnvironmentalHealth #PublicWellBeing #PathogenicBacteria #IndoorAirQuality #FungalSpores #AirborneViruses #MicrobialCommunities #AirPollution #HealthProtection #COVID19Detection #DataDrivenScience #EnvironmentalImpact #EcosystemHealth
Disease resistance marker screening through LAMP PCR (Loop-Mediated Isothermal Amplification Polymerase Chain Reaction) is a transformative approach in modern agriculture and plant genetics. By identifying and characterizing specific genetic markers associated with a plant's ability to resist pathogens, this technique revolutionizes crop improvement strategies. The process begins with the extraction of plant DNA, followed by the application of LAMP PCR's unique isothermal amplification process. Unlike traditional PCR methods, LAMP PCR's constant temperature amplification expedites the detection of disease resistance markers, making it a rapid and cost-effective tool. The visual detection of amplification, exhibited by loop structures, offers a user-friendly advantage, allowing for real-time identification without complex instrumentation. These markers enable the selection of plants with enhanced disease resistance traits for breeding programs, reducing the reliance on chemical interventions and promoting environmentally sustainable agricultural practices. Disease resistance marker screening has far-reaching implications, facilitating the development of crop varieties that can withstand pathogenic challenges and contributing to global food security. Moreover, the technique enhances our understanding of plant-pathogen interactions and opens doors for precision agriculture, biosecurity measures, and scientific advancements in crop protection. - BioPathogenix organization operates simple, systemized, and transparent processes that drive accountability, productivity, and external focus. Biopathogenix is committed to quality excellence, as well as providing unrivaled customer service, and achieve this by offering: ● products that meet all standard requirements. ● first class customer service. ● complete and on-time deliveries. Customers are at the heart of our business. We are committed to continual review and improvement of processes, products, and services, to meet and exceed our customers’ expectations. WE have established quality systems and monitor processes against performance targets set within business plans and objectives programs. WE will achieve our improvement targets through continual training and communication of the key business objectives to all personnel. Customer service measures are reported monthly as part of the “PROPS” commitment (ProductAvailability, Right First Time, On Time Delivery, Product Quality & Service Quality). We are committed to continual improvement of both products and processes. The framework for setting objectives lies within business planning for each part of the business, whereby targets are set based on opportunities for growth and improvement, and to eliminate and contain risks. The Management Review Meeting considers the effectiveness of these management programs outlined by this policy. 🤍biopathogenix.com (859) 444-5660 order🤍biopathogenix.com 120 Dewey Dr STE 126 Nicholasville, KY 40356 #AgTechAdvancements #PlantGenetics #CropImprovement #SustainableAgriculture #PrecisionAg #CropProtection #Biosecurity #FoodSecurity #PlantPathogens #GeneticMarkers #LAMPPCR #DiseaseResistance #AgriculturalInnovations #EnvironmentalSustainability #CropBreeding
LAMP PCR (Loop-mediated Isothermal Amplification Polymerase Chain Reaction) is a molecular biology technique that can be utilized for water quality assessment. It offers several advantages over traditional PCR methods, including rapid amplification, high sensitivity, and the ability to operate at a constant temperature without the need for a thermocycler. Here are some specific applications of LAMP PCR in water quality checks: Detection of Pathogens: LAMP PCR can be used to identify pathogenic microorganisms in water, such as bacteria, viruses, and parasites. It enables the rapid and sensitive detection of these pathogens, allowing for early intervention and prevention of waterborne diseases. Monitoring of Indicator Species: Certain organisms or groups of organisms can serve as indicators of water quality. For example, the presence of coliform bacteria indicates fecal contamination and the potential presence of harmful pathogens. LAMP PCR can target specific genetic markers associated with indicator species, providing a quick and accurate assessment of water quality. Identification of Harmful Algal Blooms: Harmful algal blooms (HABs) can cause significant ecological damage and pose risks to human and aquatic life. LAMP PCR can detect and differentiate algal species responsible for HABs, such as cyanobacteria (blue-green algae). Early identification of HABs allows for timely management and mitigation strategies. Monitoring of Waterborne Pathogen Sources: LAMP PCR can aid in tracing the source of waterborne pathogens by identifying specific genetic markers unique to different sources. This information is crucial for identifying contamination origins and implementing appropriate remediation measures. Detection of Environmental Contaminants: LAMP PCR can be adapted to detect various environmental contaminants, including heavy metals, pesticides, and pharmaceutical residues. By targeting specific genes associated with the contaminants, LAMP PCR offers a sensitive and reliable method for monitoring water quality in contaminated areas. Quantification of Microbial Load: LAMP PCR can also be utilized for quantifying the microbial load in water samples. By targeting specific genetic markers and amplifying them, it enables the estimation of the abundance of particular microorganisms. This information is valuable for assessing the overall microbial quality of water. Overall, LAMP PCR is a powerful tool in water quality analysis, providing rapid and accurate detection of various microorganisms, pathogens, and contaminants. Its simplicity, speed, and sensitivity make it well-suited for on-site testing and monitoring applications, facilitating timely interventions and ensuring the safety of water resources. - BioPathogenix offers turnkey solutions for laboratories as a trusted and expert service provider. We are dedicated to providing top-notch customer service, high-quality products, complete and timely deliveries, and continuous improvement in all aspects of our business. Our commitment to customer satisfaction drives our process, product, and service reviews and improvements. CONTACT (859) 444-5660 order🤍biopathogenix.com 120 Dewey Dr STE 126 Nicholasville, KY 40356 #BioPathogenix #laboratorysolutions #customerservice #highqualityproducts #timelydeliveries #continuousimprovement #expertserviceprovider #trustedpartner #customersatisfaction #processimprovement #productimprovement #servicesimprovement
Viewers! A detailed talk ..Exclusive by Mrs.Annie Florance, Industry expert and Senior Scientific Officer at Genei Laboratories, Bangalore for the benefit of students learning skill development course and Quality control..
안녕하세요 아이리스바이오입니다. 동물용 현장진단용(POCT) 분자진단키트 1SHot PCR 사용방법 영상[분변검체용]입니다.
Dr. Brian Baker from LLNL presents key concepts for developing a loop-mediated isothermal amplification (LAMP)-based pathogen nucleic acid detection system for point-of-care applications.
#RT #lamp #sars-cov-2 #stranddisplacement A técnica da transcrição reversa seguida de amplificação isotérmica mediada por laço (RT-LAMP, do inglês Loop-mediated isothermal amplification) pode ser um "game changer" na testagem da COVID-19. Vamos entender como o deslocamento de fita (strand displacement) de uma polimerase que funciona em uma reação isotérmica permite isso? 00:00 Introdução 02:49 DNA Polimerase e atividade 5'-3' 08:43 Reação de LAMP 18:30 Reação e detecção dos produtos 23:02 Detecção colorimétrica 28:37 Transcrição Reversa seguida de LAMP Bons estudos!
How to use the NAOR LAMP PCR test: point-of-care, requires only saliva swab, and returns results in 30 minutes!
GenePro LAMP Cycler is a real-time gene amplification device used to check the presence of a target gene by measuring the degree of amplification, using Loop-mediated Isothermal Amplification (LAMP). In the LAMP method, four or more primers are combined by selecting specific regions from the target gene (DNA or RNA) to create a loop structure and the amplification enzyme DNA polymerase is used to conduct a loop-mediated reaction at a constant temperature (60 - 65℃). #nanobiolife #gencurix #LAMP #ivd #ivdd
Nathan Tanner describes a method for a visually robust readout of amplification techniques, which has applications in the field for the detection of pathogens.
Step-by-step video illustration of the high throughput malaria sample processing kit for loop-mediated isothermal amplification (LAMP).
For more information, please visit our digital PCR learning center : 🤍gene-pi.com One of the greatest challenges in molecular biology is the detection of a rare allele or mutant sequence lost in a dense background of wild-type sequences. This situation is often encountered in oncology for example when trying to detect tumor-derived mutant sequences, present in very small amounts in liquid biopsies. In this animation, the mutant sequence is colored in red and the wild-type is colored in green. Finding the mutant sequence is a typical needle in a haystack problem! When using standard real-time quantitative PCR, the mixture of DNA molecules is amplified in bulk in a reaction mixture containing PCR reagents, including a Taq polymerase, PCR primers and two types of probes, each specific for the wild-type or the mutant sequence respectively. The wild-type probe is labeled with a green fluorophore, the mutant probe is labeled with a red fluorophore. During the PCR amplification, the probes specific for the mutant and wild-type sequences anneal to their respective targets and release a fluorescence signal. However, in a bulk reaction, the signal of the mutant target is in competition with the signal of the wild-type one, and when the mutant sequence is present at a very low fraction, it may not even be detected. To overcome this problem, Digital PCR relies on a basic principle: partitioning the sample prior to PCR amplification in order to isolate individual DNA molecules in different compartments. Today, several techniques are employed to achieve the partitioning. This is either done in solid microchambers or done in an emulsion of microdroplets. When the sample is partitioned, all DNA molecules are randomly distributed into a large number of partitions, such that only one or a limited number of DNA molecules end up in each partition. Even if mutant and wild-type sequences end up in the same droplet, the competition between the two is greatly reduced. During the PCR amplification, a fluorescence signature is generated within each partition according to its DNA content. One targeted DNA molecule is sufficient to generate a fluorescence signal in a given partition. Finally, the concentration of wild-type and mutant sequences in the sample is precisely estimated by counting the number of each type of partitions, classified according to the measured fluorescence levels: the non-fluorescent negatives, the greens,… the reds… and the double positives. Digital PCR also enables the simultaneous detection of multiple targets using a multiplex assay. Rigorously, a formula based on the Poisson law converts the counted negative and positive partitions into the target concentration of both the wild-type and mutant sequences. Digital PCR is a powerful tool for the detection of rare events but can also benefit other assays such as copy number variation, by being able to precisely measure less than 2-fold differences in copy number, or absolute quantification of nucleic acids, since this technique does not have to rely on comparison with precalibrated standards. Digital PCR is also a great tool for genotyping single cells. All those assays are currently applied to a wide range of applications such as oncology, infectious diseases, environmental testing, prenatal diagnosis, organ transplant, etc. How do you want to use digital PCR?
Shingo's Presentation at Virtually Maker Faire 2020 Google Presentation Link 🤍 We're fighting with COVID-19 by upgrading NinjaPCR into affordable Real-Time PCR. 🤍 Donation via PayPal 🤍 Patreon 🤍
Just a brief explanation about procedure of LAMP Test.
Product Features Easy: All reactions are taken place in the same tube. Easy to operate, learn, understand, and complex training is not required. Isothermal: Saving equipment cost. High Specificity: Detection accuracy as high as 99%+. Rapid Results:The detection can be completed within 30 minutes. Convenient Transport&Storage :Under normal temperature to transport and store.(Below 30℃
안녕하세요 아이리스바이오입니다. 동물용 현장진단용(POCT) 분자진단키트 1SHot PCR 사용방법 영상[혈액검체용]입니다.
Comparing PCR to RT-LAMP for RNA Detection and The Importance for Hop Latent Viroid Professor DeBacco PCR- Polymerase Chain Reaction Has primers that are specific to a nucleic acid region and if present and a positive binding occurs it will be copied multiple times, exponentially producing the original sequence This can not only determine if the sequence is present or not, but with other detective instrumentation the severity (how many particles were present at the start). Low or high viral (or viroid) load PCR- Thermocycler Takes “one” piece of DNA and makes many, many, many copies of it. Often called a thermos cycler as to maximize the replication cycle temperature is changed to allow the Taq polymerase to effectively bind and replication the strand. PCR Testing This molecular testing has to be performed if detection of a viroid is the goal This can also work for viruses. RT-LAMP- Reverse Transcription Loop-mediated Isothermal Amplification Loop-mediated isothermal amplification (LAMP) is a DNA amplification method that allows rapid and sensitive detection of a specific gene. LAMP merged with reverse transcription (RT-LAMP) has been successfully used for the detection of several respiratory RNA viruses. Uses a different enzyme than PCR that offers the advantage of being effective at one temperature and not a cycling of temperatures. Field Testing RT-Lamp: Similar to PCR that uses a specialize enzyme to amplify the viroid if present. Downfall- Less sensitive Good at finding positives, not as good at detecting true negatives Antigen Testing This tests for proteins so it can not be used for Viroids (unlike viruses) Which One Should I Choose? PCR is the standard for a reason and is considered to be the most reliable and also provides quick results. It does not need a high number of viroids present in the original sample (technically it would only need one present) and it would still come back with a positive indication. Link to Lecture Slides: 🤍 *Due to the description character limit the full work cited for "Comparing PCT to RT LAMP for RNA Detection and the Important for Hop Latent Viroid" can be viewed at... 🤍
Ryo Kubota, CEO Diagenetic, Inc.
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![Loop Mediated Isothermal Amplification [LAMP]: Introduction and Basics](https://i.ytimg.com/vi/OImoO0JMjbI/mqdefault.jpg "Loop Mediated Isothermal Amplification [LAMP]: Introduction and Basics")
![[아이리스바이오] 1SHot LAMP PCR 사용방법-분변검체 / 동물용 POCT분자진단키트](https://i.ytimg.com/vi/vYiaDFEeugE/mqdefault.jpg "[아이리스바이오] 1SHot LAMP PCR 사용방법-분변검체 / 동물용 POCT분자진단키트")
![[아이리스바이오] 1SHot LAMP PCR 사용방법-혈액검체 / 동물용 POCT분자진단키트](https://i.ytimg.com/vi/0bZcqJkQihI/mqdefault.jpg "[아이리스바이오] 1SHot LAMP PCR 사용방법-혈액검체 / 동물용 POCT분자진단키트")
